{"id":11730,"date":"2025-11-14T21:16:51","date_gmt":"2025-11-14T20:16:51","guid":{"rendered":"https:\/\/rvh-synergie.org\/actualites\/accelerer-la-detection-de-trois-infections-fongiques-regionales-graves\/"},"modified":"2025-11-14T21:16:55","modified_gmt":"2025-11-14T20:16:55","slug":"accelerer-la-detection-de-trois-infections-fongiques-regionales-graves","status":"publish","type":"post","link":"https:\/\/rvh-synergie.org\/actualites\/accelerer-la-detection-de-trois-infections-fongiques-regionales-graves\/","title":{"rendered":"Acc\u00e9l\u00e9rer la d\u00e9tection de trois infections fongiques r\u00e9gionales graves"},"content":{"rendered":"
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Certaines infections fongiques graves surviennent dans des r\u00e9gions des \u00c9tats-Unis pr\u00e9sentant des environnements sp\u00e9cifiques et sont souvent li\u00e9es \u00e0 l’exposition du sol. Ces infections peuvent toucher aussi bien les personnes saines que les personnes immunod\u00e9prim\u00e9es, mais le diagnostic correct reste lent, ce qui retarde le traitement.<\/p>\n

L\u2019\u00e9talon-or actuel pour \u00e9tablir un diagnostic repose sur la culture fongique, qui peut prendre des semaines, et sur des tests qui recherchent des antig\u00e8nes, qui manquent de sp\u00e9cificit\u00e9, ou des anticorps dans le sang, qui sont souvent peu fiables au d\u00e9but de la maladie. Mais maintenant, des chercheurs de l’Indiana University Health et de l’IU School of Medicine ont d\u00e9velopp\u00e9 un nouveau test mol\u00e9culaire capable de d\u00e9tecter simultan\u00e9ment trois champignons pathog\u00e8nes majeurs, et ce avec un d\u00e9lai d’ex\u00e9cution beaucoup plus rapide que les m\u00e9thodes traditionnelles.<\/p>\n

Le travail a \u00e9t\u00e9 pr\u00e9sent\u00e9 lors de la r\u00e9union annuelle et de l\u2019exposition 2025 de l\u2019Association for Molecular Pathology (AMP), qui s\u2019est tenue du 11 au 15 novembre \u00e0 Boston.<\/p>\n

Le Dr Kenneth Gavina, Ph.D., est directeur de la microbiologie clinique \u00e0 Eskanazi Health \u00e0 Indianapolis. Il a supervis\u00e9 le projet par l\u2019interm\u00e9diaire de la facult\u00e9 de m\u00e9decine de l\u2019Universit\u00e9 d\u2019Indiana. Son \u00e9quipe a cherch\u00e9 \u00e0 identifier trois infections fongiques qui touchent des milliers de personnes chaque ann\u00e9e, sont souvent confondues avec d’autres maladies respiratoires et peuvent mettre la vie en danger, en particulier chez les personnes immunod\u00e9prim\u00e9es\u00a0:<\/p>\n

    \n
  • Histoplasmose<\/b> se pr\u00e9sente g\u00e9n\u00e9ralement sous la forme d’une infection pulmonaire chez les patients qui ont inhal\u00e9 de la terre contenant des excr\u00e9ments d’oiseaux ou de chauves-souris. Il est plus courant dans les vall\u00e9es des rivi\u00e8res Ohio et Mississippi.<\/li>\n
  • Blastomycose<\/b> se pr\u00e9sente \u00e9galement comme une infection pulmonaire, mais peut \u00e9galement \u00e9voluer pour affecter la peau, les os et d\u2019autres organes. On le trouve principalement dans le centre et le sud-est des \u00c9tats-Unis.<\/li>\n
  • Coccidio\u00efdomycose<\/b> est commun\u00e9ment connue sous le nom de fi\u00e8vre de la vall\u00e9e. Bien que la plupart de ces infections pulmonaires soient b\u00e9nignes, certains patients d\u00e9veloppent une pneumonie grave ou souffrent d’une infection ailleurs dans le corps. On le trouve principalement dans les \u00c9tats du sud-ouest, notamment en Arizona, en Californie et au Nouveau-Mexique.<\/li>\n<\/ul>\n

    Les chercheurs ont con\u00e7u un test PCR multiplex en temps r\u00e9el, ce qui signifie qu\u2019il peut d\u00e9tecter simultan\u00e9ment plusieurs agents pathog\u00e8nes \u00e0 partir du m\u00eame \u00e9chantillon.<\/p>\n

    Leur test a cibl\u00e9 trois r\u00e9gions g\u00e9n\u00e9tiques uniques \u00e0 chaque champignon : ITS1 pour Histoplasma, BAD1 pour Blastomyces et le g\u00e8ne A2\/PRA pour Coccidioides. En d\u00e9tectant directement le mat\u00e9riel g\u00e9n\u00e9tique des champignons, le test PCR contourne le lent processus de culture compliqu\u00e9 par la capacit\u00e9 des champignons \u00e0 basculer entre les formes environnementales et humaines.<\/p>\n

    Lorsqu\u2019ils ont compar\u00e9 leurs r\u00e9sultats PCR aux m\u00e9thodes de laboratoire standard, le test PCR a identifi\u00e9 tous les \u00e9chantillons test\u00e9s avec une pr\u00e9cision de 100 %. De plus, il \u00e9tait sp\u00e9cifique \u00e0 100 %, ce qui signifie qu\u2019il ne d\u00e9tectait pas par erreur d\u2019autres champignons ou contaminants.<\/p>\n

    Le flux de travail \u00e9limine \u00e9galement le besoin d\u2019\u00e9tapes fastidieuses d\u2019extraction de l\u2019ADN, ce qui r\u00e9duit potentiellement les d\u00e9lais d\u2019ex\u00e9cution et les risques en laboratoire associ\u00e9s \u00e0 la manipulation de champignons pathog\u00e8nes vivants.<\/p>\n

    \u00ab\u00a0Notre test a le potentiel d’am\u00e9liorer consid\u00e9rablement les d\u00e9lais d’ex\u00e9cution et la fiabilit\u00e9 du diagnostic pour les infections qui ont toujours \u00e9t\u00e9 difficiles \u00e0 d\u00e9tecter rapidement\u00a0\u00bb, a d\u00e9clar\u00e9 Gavina.<\/p>\n

    Il est important de noter que le syst\u00e8me de l’\u00e9quipe fonctionne sur une plateforme d\u00e9j\u00e0 utilis\u00e9e dans de nombreux laboratoires cliniques, ce qui le rend potentiellement tr\u00e8s accessible.<\/p>\n

    \u00ab\u00a0Bien qu’une validation clinique suppl\u00e9mentaire soit en cours, ce test montre un fort potentiel pour combler une lacune majeure dans le diagnostic fongique\u00a0\u00bb, a d\u00e9clar\u00e9 Gavina. Le test a la capacit\u00e9 de d\u00e9tecter les trois principales causes de mycoses end\u00e9miques aux \u00c9tats-Unis directement \u00e0 partir d’\u00e9chantillons cliniques ou d’\u00eatre utilis\u00e9 comme forme d’identification d\u00e9finitive \u00e0 partir d’une culture fongique.<\/p>\n

    \u00ab\u00a0Les cliniciens ne disposent actuellement d’aucun test mol\u00e9culaire approuv\u00e9 par la FDA pour ces trois agents pathog\u00e8nes, ce qui rend une d\u00e9tection rapide et fiable un besoin urgent.\u00a0\u00bb<\/p>\n

    \t\t\t\t\t\t\t\t\t\t\t\tFourni par l’Association pour la pathologie mol\u00e9culaire<\/p>\n

    <\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

    Certaines infections fongiques graves surviennent dans des r\u00e9gions des \u00c9tats-Unis pr\u00e9sentant des environnements sp\u00e9cifiques et<\/p>\n","protected":false},"author":1,"featured_media":11731,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86],"tags":[1535,2366,405,581,607,5378,551],"class_list":["post-11730","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-les-maladies","tag-accelerer","tag-detection","tag-fongiques","tag-graves","tag-infections","tag-regionales","tag-trois","generate-columns","tablet-grid-50","mobile-grid-100","grid-parent","grid-50","resize-featured-image"],"yoast_head":"\nAcc\u00e9l\u00e9rer la d\u00e9tection de trois infections fongiques r\u00e9gionales graves - RVH-Synergie.org<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/rvh-synergie.org\/actualites\/accelerer-la-detection-de-trois-infections-fongiques-regionales-graves\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Acc\u00e9l\u00e9rer la d\u00e9tection de trois infections fongiques r\u00e9gionales graves - 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